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scholarly-writing-workbench/review-outline.md
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# AI-Driven Design of 16-Membered Macrolides: From Traditional Antibiotic Optimization to Intelligent Molecular Generation
This file is the default primary outline for the current project.
Each new task round should read this file first to determine the target chapter and subsection.
If this file is missing or clearly outdated, an agent may extract a provisional outline from the Word draft and write:
- `review-outline.generated.md`
Once the generated outline is manually confirmed, it should be folded back into this file.
---
## Abstract
## Chapter 1. Introduction
### 1.1 Development and challenges of antibiotics
#### 1.1.1 Overview
#### 1.1.2 Macrolide antibiotics
#### 1.1.3 Current research status of macrolide antibiotics
### 1.2 Drug resistance in macrolides
#### 1.2.1 Resistance mechanisms of macrolide antibiotics
#### 1.2.2 Binding sites of macrolide antibiotics
### 1.3 Quantitative structure-activity relationships
#### 1.3.1 Molecular representation
#### 1.3.2 1D-QSAR
#### 1.3.3 2D-QSAR
#### 1.3.4 3D-QSAR
### 1.4 Molecular energy minimization
#### 1.4.1 Molecular mechanics
#### 1.4.2 Quantum mechanics
#### 1.4.3 Hybrid quantum mechanics / molecular mechanics (QM/MM)
#### 1.4.4 Conformational ensemble sampling
### 1.5 AI-based innovative antibiotic design
## Chapter 2. Current status of macrocycle design methods
### 2.1 Evolution of computer-aided macrocycle design
#### 2.1.1 Early geometric matching and fragment stitching
#### 2.1.2 Structured fragment-linking algorithms
#### 2.1.3 Commercial and semi-automated tools
#### 2.1.4 LigMac: end-to-end structure-guided design
#### 2.1.5 Molecular-field-based fragment replacement tools
#### 2.1.6 Web platforms with configurable linker libraries
### 2.2 Generative deep learning for macrocycle design
### 2.3 Reinforcement learning optimization for macrocycle design
## Chapter 3. Specialized models and strategies for macrocycle generation
### 3.1 Generative models based on fragment linking / cyclization
### 3.2 Generative models for macrocyclic peptides and special scaffolds
### 3.3 Generative models and tools for macrolides
#### 3.3.1 PKS-based generation of macrolides
## Chapter 4. AI-driven molecular generation techniques
### 4.1 Sequence-based generative models (SMILES representation)
### 4.2 Molecular graph-based generative models
### 4.3 GAN and reinforcement learning methods
### 4.4 Emerging diffusion models and 3D generation
## Chapter 5. Generative models for macrocyclic molecules
### 5.1 Generative models for macrocycles
#### 5.1.1 Challenges in macrocycle design
#### 5.1.2 Macformer: macrocycle structure generation
#### 5.1.3 MacroHop: macrocycle scaffold generation
#### 5.1.4 MacroEvoLution: evolutionary macrocycle design
#### 5.1.5 HELM-GPT: macrocyclic peptide generation
### 5.2 Specialized tools for macrolides
#### 5.2.1 PKS Enumerator
#### 5.2.2 SIME: biosynthesis-inspired macrocycle design
#### 5.2.3 Biosynthesis-driven macrocycle design strategies
### 5.3 Fixed-scaffold structure generation strategies
#### 5.3.1 Scaffold-constrained generation
#### 5.3.2 Site-directed generation
#### 5.3.3 Fragment stitching and side-chain enumeration
## Conclusion and outlook
## References