diff --git a/backend/app/workers/tasks.py b/backend/app/workers/tasks.py
index a5b818f..ac3edfc 100644
--- a/backend/app/workers/tasks.py
+++ b/backend/app/workers/tasks.py
@@ -160,6 +160,10 @@ def run_bttoxin_analysis(
                             if file_path == zip_path:
                                 continue
 
+                            # 防止嵌套打包：忽略可能存在的 zip 或 tar.gz 文件
+                            if file.endswith('.zip') or file.endswith('.tar.gz'):
+                                continue
+
                             # 计算相对路径，例如 digger/Results/foo.txt -> Results/foo.txt
                             rel_path = file_path.relative_to(src_path)
                             # 构造新的归档路径 -> 1_Toxin_Mining/Results/foo.txt
diff --git a/docker-start.sh b/docker-start.sh
old mode 100755
new mode 100644
diff --git a/docker-stop.sh b/docker-stop.sh
old mode 100755
new mode 100644
diff --git a/docker/scripts/entrypoint.sh b/docker/scripts/entrypoint.sh
old mode 100755
new mode 100644
diff --git a/docker/scripts/switch-to-traefik.sh b/docker/scripts/switch-to-traefik.sh
old mode 100755
new mode 100644
diff --git a/pixi.lock b/pixi.lock
index 9632cfe..ab845c4 100644
--- a/pixi.lock
+++ b/pixi.lock
@@ -4,26 +4,25 @@ environments:
     channels:
     - url: https://conda.anaconda.org/conda-forge/
     - url: https://conda.anaconda.org/bioconda/
-    - url: https://conda.anaconda.org/bioconda/label/cf201901/
     options:
       channel-priority: disabled
-      pypi-prerelease-mode: if-necessary-or-explicit
     packages:
       linux-64:
       - conda: https://conda.anaconda.org/conda-forge/linux-64/_libgcc_mutex-0.1-conda_forge.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-2_gnu.tar.bz2
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/alsa-lib-1.2.15.3-hb03c661_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/biopython-1.86-py314h5bd0f2a_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-1.2.0-hed03a55_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-bin-1.2.0-hb03c661_1.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/blast-2.17.0-h66d330f_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_8.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/c-ares-1.34.6-hb03c661_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.1.4-hbd8a1cb_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/cairo-1.18.4-he90730b_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/contourpy-1.3.3-py314h9891dd4_3.conda
-      - conda: https://conda.anaconda.org/conda-forge/noarch/cycler-0.12.1-pyhcf101f3_2.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/cyrus-sasl-2.1.28-hd9c7081_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/dbus-1.16.2-h24cb091_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/double-conversion-3.4.0-hecca717_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.1.4-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/colorama-0.4.6-pyhd8ed1ab_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/colorlog-6.10.1-pyh707e725_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/cpp-expected-1.3.1-h171cf75_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/curl-8.18.0-h4e3cde8_0.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/emboss-6.6.0-h0f19ade_14.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/entrez-direct-24.0-he881be0_0.tar.bz2
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.7.3-hecca717_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/fmt-12.1.0-hff5e90c_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
@@ -31,144 +30,141 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.15.0-h7e30c49_1.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-ecosystem-1-0.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/noarch/fonttools-4.61.1-pyh7db6752_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/freetype-2.14.1-ha770c72_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/graphite2-1.3.14-hecca717_2.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/harfbuzz-12.3.0-h6083320_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/git-2.51.0-pl5321h28ef92a_0.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/hmmer-3.3.2-hdbdd923_4.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/icu-78.2-h33c6efd_0.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/java-jdk-8.0.112-1.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/keyutils-1.6.3-hb9d3cd8_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/kiwisolver-1.4.9-py314h97ea11e_2.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/krb5-1.21.3-h659f571_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/lcms2-2.18-h0c24ade_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/ld_impl_linux-64-2.45-default_hbd61a6d_105.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/lerc-4.0.0-h0aef613_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libarchive-3.8.5-gpl_hc2c16d8_100.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libblas-3.11.0-5_h4a7cf45_openblas.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libbrotlicommon-1.2.0-hb03c661_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libbrotlidec-1.2.0-hb03c661_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libbrotlienc-1.2.0-hb03c661_1.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libcblas-3.11.0-5_h0358290_openblas.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libclang-cpp21.1-21.1.8-default_h99862b1_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libclang13-21.1.8-default_h746c552_1.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libcups-2.3.3-hb8b1518_5.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libcurl-8.18.0-h4e3cde8_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libdb-6.2.32-h9c3ff4c_0.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libdeflate-1.25-h17f619e_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libdrm-2.4.125-hb03c661_1.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libedit-3.1.20250104-pl5321h7949ede_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libegl-1.7.0-ha4b6fd6_2.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libev-4.33-hd590300_2.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.7.3-hecca717_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.5.2-h9ec8514_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype-2.14.1-ha770c72_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype6-2.14.1-h73754d4_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-15.2.0-he0feb66_16.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-ng-15.2.0-h69a702a_16.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libgd-2.3.3-h5fbf134_12.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libgfortran-15.2.0-h69a702a_16.conda
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-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libglib-2.86.3-h6548e54_0.conda
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       - conda: https://conda.anaconda.org/conda-forge/linux-64/libgomp-15.2.0-he0feb66_16.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libharu-2.4.5-h943b412_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libiconv-1.18-h3b78370_2.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libidn2-2.3.8-hfac485b_1.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libjpeg-turbo-3.1.2-hb03c661_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/liblapack-3.11.0-5_h47877c9_openblas.conda
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-      - conda: https://conda.anaconda.org/conda-forge/linux-64/liblzma-5.8.1-hb9d3cd8_2.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libmpdec-4.0.0-hb9d3cd8_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libntlm-1.8-hb9d3cd8_0.conda
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+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libnghttp2-1.67.0-had1ee68_0.conda
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       - conda: https://conda.anaconda.org/conda-forge/linux-64/libopenblas-0.3.30-pthreads_h94d23a6_4.conda
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-      - conda: https://conda.anaconda.org/conda-forge/linux-64/libpq-18.1-hb80d175_3.conda
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       - conda: https://conda.anaconda.org/conda-forge/linux-64/libsqlite-3.51.2-hf4e2dac_0.conda
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       - conda: https://conda.anaconda.org/conda-forge/linux-64/libtiff-4.7.1-h9d88235_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/libunistring-0.9.10-h7f98852_0.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/libuuid-2.41.3-h5347b49_0.conda
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+  - perl-list-moreutils
+  - zlib
+  license: NCBI-PD
+  size: 84832339
+  timestamp: 1754909742570
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   license: MIT
   license_family: MIT
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   timestamp: 1765062031645
+- conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.10.19-h3c07f61_2_cpython.conda
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+  - tzdata
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+  license: Python-2.0
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 - conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.14.2-h32b2ec7_100_cp314.conda
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   timestamp: 1761503229008
-- conda: https://conda.anaconda.org/conda-forge/noarch/python-multipart-0.0.21-pyhcf101f3_1.conda
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-  md5: 6881f5cd0aae736cc1ab21354c83ec49
+- conda: https://conda.anaconda.org/conda-forge/noarch/python-multipart-0.0.22-pyhcf101f3_0.conda
+  sha256: 8275c88b0f138dbd602c53bae9a11789126c6a2c97f7e89f679d3e7ccbb121ba
+  md5: 5a2610edf297cbd1cbc0e2c17bc47efc
   depends:
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   - python
   license: Apache-2.0
-  license_family: APACHE
-  size: 30275
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+  size: 30342
+  timestamp: 1769356329419
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-tzdata-2025.3-pyhd8ed1ab_0.conda
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   md5: 7ead57407430ba33f681738905278d03
@@ -6787,6 +7452,16 @@ packages:
   license_family: BSD
   size: 6388
   timestamp: 1695147335408
+- conda: https://conda.anaconda.org/conda-forge/noarch/python_abi-3.10-8_cp310.conda
+  build_number: 8
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+  md5: 05e00f3b21e88bb3d658ac700b2ce58c
+  constrains:
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+  license: BSD-3-Clause
+  license_family: BSD
+  size: 6999
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 - conda: https://conda.anaconda.org/conda-forge/noarch/python_abi-3.14-8_cp314.conda
   build_number: 8
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@@ -6816,6 +7491,19 @@ packages:
   license_family: BSD
   size: 4856
   timestamp: 1646866525560
+- conda: https://conda.anaconda.org/conda-forge/linux-64/pyyaml-6.0.3-py310h3406613_0.conda
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+  - __glibc >=2.17,<3.0.a0
+  - libgcc >=14
+  - python >=3.10,<3.11.0a0
+  - python_abi 3.10.* *_cp310
+  - yaml >=0.2.5,<0.3.0a0
+  license: MIT
+  license_family: MIT
+  size: 180966
+  timestamp: 1758892005321
 - conda: https://conda.anaconda.org/conda-forge/noarch/pyyaml-6.0.3-pyh7db6752_0.conda
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@@ -6982,6 +7670,28 @@ packages:
   license_family: MIT
   size: 228208
   timestamp: 1763575121128
+- conda: https://conda.anaconda.org/conda-forge/linux-64/reproc-14.2.5.post0-hb9d3cd8_0.conda
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+  md5: 69fbc0a9e42eb5fe6733d2d60d818822
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+  - libgcc >=13
+  license: MIT
+  license_family: MIT
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+  timestamp: 1731925834928
+- conda: https://conda.anaconda.org/conda-forge/linux-64/reproc-cpp-14.2.5.post0-h5888daf_0.conda
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+  depends:
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+  - libgcc >=13
+  - libstdcxx >=13
+  - reproc 14.2.5.post0 hb9d3cd8_0
+  license: MIT
+  license_family: MIT
+  size: 25665
+  timestamp: 1731925852714
 - conda: https://conda.anaconda.org/conda-forge/noarch/requests-2.32.5-pyhcf101f3_1.conda
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   size: 63602
   timestamp: 1766926974520
-- conda: https://conda.anaconda.org/conda-forge/noarch/rich-14.2.0-pyhcf101f3_0.conda
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-  md5: a247579d8a59931091b16a1e932bbed6
+- conda: https://conda.anaconda.org/conda-forge/noarch/rich-14.3.1-pyhcf101f3_0.conda
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+  md5: 83d94f410444da5e2f96e5742b7a4973
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   - python
   license: MIT
-  license_family: MIT
-  size: 200840
-  timestamp: 1760026188268
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+  timestamp: 1769302653091
 - conda: https://conda.anaconda.org/conda-forge/noarch/rich-toolkit-0.17.1-pyhcf101f3_0.conda
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-- conda: https://conda.anaconda.org/conda-forge/linux-64/scipy-1.16.3-py314hf07bd8e_2.conda
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-  - libgfortran
-  - libgfortran5 >=14.3.0
-  - liblapack >=3.9.0,<4.0a0
-  - libstdcxx >=14
-  - numpy <2.6
-  - numpy >=1.23,<3
-  - numpy >=1.25.2
-  - python >=3.14,<3.15.0a0
-  - python_abi 3.14.* *_cp314
-  license: BSD-3-Clause
-  license_family: BSD
-  size: 16982488
-  timestamp: 1766108668132
-- conda: https://conda.anaconda.org/conda-forge/linux-64/scipy-1.17.0-py314hf07bd8e_0.conda
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-  md5: 2d82ddc8e7a74d27382410462df062a2
+- conda: https://conda.anaconda.org/conda-forge/linux-64/scipy-1.17.0-py314hf07bd8e_1.conda
+  sha256: a95de421c586de901402107fbeb7524efaee5bb55c1aba2e1334f8b8ebc89093
+  md5: c7df812186fb1290bc00d9b7b5a50b18
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   - __glibc >=2.17,<3.0.a0
   - libblas >=3.9.0,<4.0a0
@@ -7098,8 +7786,8 @@ packages:
   - python_abi 3.14.* *_cp314
   license: BSD-3-Clause
   license_family: BSD
-  size: 16919236
-  timestamp: 1768135976439
+  size: 17048277
+  timestamp: 1768800950735
 - conda: https://conda.anaconda.org/conda-forge/noarch/seaborn-0.13.2-hd8ed1ab_3.conda
   noarch: python
   sha256: ea29a69b14dd6be5cdeeaa551bf50d78cafeaf0351e271e358f9b820fcab4cb0
@@ -7135,6 +7823,17 @@ packages:
   license_family: MIT
   size: 15018
   timestamp: 1762858315311
+- conda: https://conda.anaconda.org/conda-forge/linux-64/simdjson-4.2.4-hb700be7_0.conda
+  sha256: ffe0c49e65486b485e66c7e116b1782189c970c16cb2fe9710a568e44bb9ede3
+  md5: da6caa4c932708d447fb80eed702cb4e
+  depends:
+  - __glibc >=2.17,<3.0.a0
+  - libgcc >=14
+  - libstdcxx >=14
+  license: Apache-2.0
+  license_family: APACHE
+  size: 294996
+  timestamp: 1766034103379
 - conda: https://conda.anaconda.org/conda-forge/noarch/six-1.17.0-pyhe01879c_1.conda
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-- conda: https://conda.anaconda.org/conda-forge/linux-64/sqlalchemy-2.0.45-py314h5bd0f2a_0.conda
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-  md5: 7f6f4f594460dd21e3e61a0c90fef4c9
+- conda: https://conda.anaconda.org/conda-forge/linux-64/spdlog-1.17.0-hab81395_1.conda
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+  md5: a3b0e874fa56f72bc54e5c595712a333
   depends:
   - __glibc >=2.17,<3.0.a0
-  - greenlet !=0.4.17
+  - fmt >=12.1.0,<12.2.0a0
   - libgcc >=14
-  - python >=3.14,<3.15.0a0
-  - python_abi 3.14.* *_cp314
-  - typing-extensions >=4.6.0
+  - libstdcxx >=14
   license: MIT
   license_family: MIT
-  size: 3837797
-  timestamp: 1765351946542
+  size: 196681
+  timestamp: 1767781665629
+- conda: https://conda.anaconda.org/conda-forge/linux-64/sqlalchemy-2.0.46-py314h0f05182_1.conda
+  sha256: c964b16ce7b66e14963e11249e1344cacdbd5540831ead8882db013238c4b9f0
+  md5: f71a19fc80d24b3105ccc096465b5520
+  depends:
+  - python
+  - greenlet !=0.4.17
+  - typing-extensions >=4.6.0
+  - libgcc >=14
+  - __glibc >=2.17,<3.0.a0
+  - python_abi 3.14.* *_cp314
+  license: MIT
+  license_family: MIT
+  size: 4022158
+  timestamp: 1769087054772
 - conda: https://conda.anaconda.org/conda-forge/linux-64/sqlite-3.46.0-h6d4b2fc_0.conda
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@@ -7279,16 +7990,16 @@ packages:
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   timestamp: 1763054914403
-- conda: https://conda.anaconda.org/conda-forge/noarch/tomli-2.3.0-pyhcf101f3_0.conda
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-  md5: d2732eb636c264dc9aa4cbee404b1a53
+- conda: https://conda.anaconda.org/conda-forge/noarch/tomli-2.4.0-pyhcf101f3_0.conda
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+  md5: 72e780e9aa2d0a3295f59b1874e3768b
   depends:
   - python >=3.10
   - python
   license: MIT
   license_family: MIT
-  size: 20973
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 - conda: https://conda.anaconda.org/conda-forge/linux-64/tornado-6.5.3-py314h5bd0f2a_0.conda
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@@ -7504,6 +8215,16 @@ packages:
   license_family: BSD
   size: 14659
   timestamp: 1733906502297
+- conda: https://conda.anaconda.org/bioconda/linux-64/vmatch-2.3.1-h7b50bb2_0.conda
+  sha256: a87d7b92f1b96a9febc54e5c46c11638c472b2027b4aa759edbc397d4de0aef0
+  md5: 242dc1e14687c8e3a8a41473322fb5da
+  depends:
+  - libgcc >=13
+  - perl >=5.10,<6
+  license: Unknown
+  license_family: OTHER
+  size: 1267376
+  timestamp: 1758666351776
 - conda: https://conda.anaconda.org/conda-forge/linux-64/watchfiles-1.1.1-py314ha5689aa_0.conda
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@@ -7550,18 +8271,18 @@ packages:
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-- conda: https://conda.anaconda.org/conda-forge/linux-64/websockets-15.0.1-py314h31f8a6b_2.conda
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-  md5: e05c3cce47cc4f32f886eb17091ba6e2
+- conda: https://conda.anaconda.org/conda-forge/linux-64/websockets-16.0-py314h0f05182_1.conda
+  sha256: 15cf4ebe64022b71bcec9e3554c0b36f6cab4d7726018ccf768982bc3984198e
+  md5: 26fea8d00be28d4c6f6b49fe6b31cd1e
   depends:
   - python
-  - __glibc >=2.17,<3.0.a0
   - libgcc >=14
+  - __glibc >=2.17,<3.0.a0
   - python_abi 3.14.* *_cp314
   license: BSD-3-Clause
   license_family: BSD
-  size: 380425
-  timestamp: 1756476367704
+  size: 382988
+  timestamp: 1768087395103
 - conda: https://conda.anaconda.org/bioconda/linux-64/wfa2-lib-2.3.5-h9948957_3.tar.bz2
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@@ -7586,6 +8307,22 @@ packages:
   license_family: GPL
   size: 823594
   timestamp: 1632718925651
+- conda: https://conda.anaconda.org/conda-forge/linux-64/wget-1.25.0-hc2d8bac_0.conda
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+  md5: 54ef9e962b9f75026416a4ddbafbc854
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+  - __glibc >=2.17,<3.0.a0
+  - libgcc >=14
+  - libidn2 >=2,<3.0a0
+  - libunistring >=0,<1.0a0
+  - libzlib >=1.3.1,<2.0a0
+  - openssl >=3.5.4,<4.0a0
+  - pcre2 >=10.45,<10.46.0a0
+  - zlib
+  license: GPL-3.0-or-later
+  license_family: GPL
+  size: 743192
+  timestamp: 1761206260377
 - conda: https://conda.anaconda.org/conda-forge/linux-64/xcb-util-0.4.0-h166bdaf_0.tar.bz2
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@@ -8100,6 +8837,18 @@ packages:
   license_family: MIT
   size: 85189
   timestamp: 1753484064210
+- conda: https://conda.anaconda.org/conda-forge/linux-64/yaml-cpp-0.8.0-h3f2d84a_0.conda
+  sha256: 4b0b713a4308864a59d5f0b66ac61b7960151c8022511cdc914c0c0458375eca
+  md5: 92b90f5f7a322e74468bb4909c7354b5
+  depends:
+  - libstdcxx >=13
+  - libgcc >=13
+  - __glibc >=2.17,<3.0.a0
+  - libgcc >=13
+  license: MIT
+  license_family: MIT
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 - conda: https://conda.anaconda.org/conda-forge/linux-64/zlib-1.2.13-h4ab18f5_6.conda
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@@ -8110,6 +8859,17 @@ packages:
   license_family: Other
   size: 92883
   timestamp: 1716874088980
+- conda: https://conda.anaconda.org/conda-forge/linux-64/zlib-1.3.1-hb9d3cd8_2.conda
+  sha256: 5d7c0e5f0005f74112a34a7425179f4eb6e73c92f5d109e6af4ddeca407c92ab
+  md5: c9f075ab2f33b3bbee9e62d4ad0a6cd8
+  depends:
+  - __glibc >=2.17,<3.0.a0
+  - libgcc >=13
+  - libzlib 1.3.1 hb9d3cd8_2
+  license: Zlib
+  license_family: Other
+  size: 92286
+  timestamp: 1727963153079
 - conda: https://conda.anaconda.org/conda-forge/linux-64/zlib-ng-2.3.2-hceb46e0_1.conda
   sha256: f2b6a175677701a0b6ce556b3bd362dc94a4e36ffcd10e3860e52ca036b4ad96
   md5: 40feea2979654ed579f1cda7c63ccb94
diff --git a/pixi.toml b/pixi.toml
index b982f3b..21b5f66 100644
--- a/pixi.toml
+++ b/pixi.toml
@@ -63,12 +63,27 @@ pytest = "*"
 #
 # 预期依赖（待激活时添加）:
 [feature.crispr.dependencies]
-python = ">=3.9"
-# crisprcasfinder = "*"  # 需要配置安装源
-biopython = "*"
-pandas = ">=2.0.0"
+python = ">=3.10"
+wget = "*"
+curl = "*"
+git = "*"
+java-jdk = "*"
+parallel = "*"
+perl-app-cpanminus = "*"
+hmmer = "*"
+emboss = "*"
+blast = "*"
+perl-bioperl-core = "*"
+perl-xml-simple = "*"
+perl-digest-md5 = "*"
+vmatch = "*"
+muscle = "*"
+prodigal = "*"
+mamba = "*"
+macsyfinder = "==2.1.2"
 
 [feature.crispr.tasks]
+install-casfinder = "macsydata install -u CASFinder==3.1.0"
 detect = "python tools/crispr_cas_analysis/scripts/detect_crispr.py"
 fusion = "python tools/crispr_cas_analysis/scripts/fusion_analysis.py"
 
@@ -80,7 +95,7 @@ digger = ["digger"]
 pipeline = ["pipeline"]
 frontend = ["frontend"]
 webbackend = ["webbackend"]
-crispr = ["crispr", "pipeline"] # Inherit pipeline to get pandas etc? Or just pipeline deps. Let's make crispr feature add to pipeline env if possible, or just use pipeline env for running these tools since they are python.
+crispr = ["crispr"]
 
 # Actually, let's keep it simple. The tools are Python scripts. They can run in the 'pipeline' environment which has python and pandas.
 # The 'crispr' feature defines dependencies.
diff --git a/scripts/download_bpprc_data.py b/scripts/download_bpprc_data.py
old mode 100755
new mode 100644
diff --git a/scripts/start_web.sh b/scripts/start_web.sh
old mode 100755
new mode 100644
diff --git a/tools/bttoxin_digger/run_digger_pixi.sh b/tools/bttoxin_digger/run_digger_pixi.sh
old mode 100755
new mode 100644
diff --git a/tools/crispr_cas_analysis/pixi.toml b/tools/crispr_cas_analysis/pixi.toml
new file mode 100644
index 0000000..9fbf57f
--- /dev/null
+++ b/tools/crispr_cas_analysis/pixi.toml
@@ -0,0 +1,30 @@
+[workspace]
+authors = ["zly <644706215@qq.com>"]
+channels = ["https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge", "https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda", "https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/main"]
+name = "crispr_cas_analysis"
+platforms = ["linux-64"]
+version = "0.1.0"
+
+[dependencies]
+python = ">=3.10"
+wget = "*"
+curl = "*"
+git = "*"
+java-jdk = "*"
+parallel = "*"
+perl-app-cpanminus = "*"
+hmmer = "*"
+emboss = "*"
+blast = "*"
+perl-bioperl-core = "*"
+perl-xml-simple = "*"
+perl-digest-md5 = "*"
+vmatch = "*"
+muscle = "*"
+prodigal = "*"
+mamba = "*"
+macsyfinder = "==2.1.2"
+
+[tasks]
+install-casfinder = "macsydata install -u CASFinder==3.1.0"
+detect = "python scripts/detect_crispr.py"
diff --git a/tools/crispr_cas_analysis/tests/__init__.py b/tools/crispr_cas_analysis/tests/__init__.py
deleted file mode 100644
index 76cf310..0000000
--- a/tools/crispr_cas_analysis/tests/__init__.py
+++ /dev/null
@@ -1 +0,0 @@
-"""Tests for CRISPR-Cas module"""
\ No newline at end of file
diff --git a/tools/crispr_cas_analysis/tests/test_detect_crispr.py b/tools/crispr_cas_analysis/tests/test_detect_crispr.py
deleted file mode 100644
index 64360e0..0000000
--- a/tools/crispr_cas_analysis/tests/test_detect_crispr.py
+++ /dev/null
@@ -1,42 +0,0 @@
-import pytest
-import json
-import shutil
-from pathlib import Path
-from crispr_cas.scripts.detect_crispr import generate_mock_results
-
-def test_generate_mock_results(tmp_path):
-    """Test mock result generation"""
-    input_file = tmp_path / "test_genome.fna"
-    input_file.touch()
-
-    results = generate_mock_results(input_file)
-
-    assert results["strain_id"] == "test_genome"
-    assert "cas_systems" in results
-    assert "arrays" in results
-    assert results["summary"]["has_cas"] is True
-    assert len(results["arrays"]) > 0
-
-def test_script_execution(tmp_path):
-    """Test full script execution via subprocess"""
-    # Create dummy input
-    input_file = tmp_path / "genome.fna"
-    input_file.touch()
-    output_file = tmp_path / "results.json"
-    script_path = Path("crispr_cas/scripts/detect_crispr.py").absolute()
-
-    import subprocess
-    cmd = [
-        "python3", str(script_path),
-        "--input", str(input_file),
-        "--output", str(output_file),
-        "--mock"
-    ]
-
-    result = subprocess.run(cmd, capture_output=True, text=True)
-    assert result.returncode == 0
-    assert output_file.exists()
-
-    with open(output_file) as f:
-        data = json.load(f)
-        assert data["strain_id"] == "genome"
diff --git a/tools/crispr_cas_analysis/tests/test_fusion_analysis.py b/tools/crispr_cas_analysis/tests/test_fusion_analysis.py
deleted file mode 100644
index 1ecdfd1..0000000
--- a/tools/crispr_cas_analysis/tests/test_fusion_analysis.py
+++ /dev/null
@@ -1,93 +0,0 @@
-import pytest
-import json
-from pathlib import Path
-import sys
-
-# Add project root to path to allow importing modules
-sys.path.insert(0, str(Path(__file__).parents[2]))
-
-from crispr_cas.scripts.fusion_analysis import calculate_distance, perform_fusion_analysis
-
-def test_calculate_distance():
-    """Test genomic distance calculation"""
-    # Same contig, no overlap
-    # Range1: 100-200, Range2: 300-400 -> Dist 100
-    assert calculate_distance("c1:100-200", "c1:300-400") == 100
-
-    # Same contig, overlap
-    # Range1: 100-300, Range2: 200-400 -> Dist 0
-    assert calculate_distance("c1:100-300", "c1:200-400") == 0
-
-    # Different contig
-    assert calculate_distance("c1:100-200", "c2:300-400") == -1
-
-    # Invalid format
-    assert calculate_distance("invalid", "c1:100-200") == -1
-
-def test_fusion_analysis_logic(tmp_path):
-    """Test main analysis logic with mock data"""
-
-    # Mock CRISPR data
-    crispr_data = {
-        "strain_id": "test_strain",
-        "arrays": [
-            {
-                "id": "A1",
-                "contig": "contig_1",
-                "start": 1000,
-                "end": 2000,
-                "spacers": [{"sequence": "ATGC"}]
-            }
-        ]
-    }
-
-    # Mock toxin file (just a placeholder for path)
-    toxin_file = tmp_path / "toxins.txt"
-    toxin_file.touch()
-
-    # Run analysis in mock mode
-    # In mock mode, the script generates its own toxin list:
-    # {"name": "Cry1Ac1", "position": "contig_1:10000-12000"}
-    # Distance: 10000 - 2000 = 8000 (< 10000 threshold) -> Should match
-
-    results = perform_fusion_analysis(crispr_data, toxin_file, mock=True)
-
-    assert results["strain_id"] == "test_strain"
-    assert len(results["associations"]) > 0
-
-    # Check for proximity match
-    proximity_matches = [a for a in results["associations"] if a["type"] == "proximity"]
-    assert len(proximity_matches) > 0
-    assert proximity_matches[0]["distance"] == 8000
-
-def test_script_execution(tmp_path):
-    """Test full script execution via subprocess"""
-
-    # Create input files
-    crispr_file = tmp_path / "crispr.json"
-    with open(crispr_file, 'w') as f:
-        json.dump({"strain_id": "test", "arrays": []}, f)
-
-    toxin_file = tmp_path / "toxins.txt"
-    toxin_file.touch()
-
-    genome_file = tmp_path / "genome.fna"
-    genome_file.touch()
-
-    output_file = tmp_path / "output.json"
-
-    script_path = Path("crispr_cas/scripts/fusion_analysis.py").absolute()
-
-    import subprocess
-    cmd = [
-        "python3", str(script_path),
-        "--crispr-results", str(crispr_file),
-        "--toxin-results", str(toxin_file),
-        "--genome", str(genome_file),
-        "--output", str(output_file),
-        "--mock"
-    ]
-
-    result = subprocess.run(cmd, capture_output=True, text=True)
-    assert result.returncode == 0
-    assert output_file.exists()