diff --git a/.claude/skills/ralph-runner/README.md b/.claude/skills/ralph-runner/README.md
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+# Ralph Runner Claude Skill
+
+Claude Skill for managing BtToxin Pipeline development with Ralph autonomous AI agent.
+
+## Directory Structure
+
+```
+ralph-runner/
+├── SKILL.md           # Main skill documentation
+├── README.md          # This file
+└── scripts/
+    ├── status.sh      # Check project and Ralph status
+    ├── start.sh       # Start Ralph loop
+    └── commit.sh      # Helper for git commits
+```
+
+## Quick Start
+
+### Option 1: Use the Skill directly
+
+When working on BtToxin Pipeline, invoke this skill to:
+- Start Ralph development loop
+- Monitor progress
+- Check status
+- Manage commits
+
+### Option 2: Use helper scripts
+
+```bash
+# Check status
+.claude/skills/ralph-runner/scripts/status.sh
+
+# Start Ralph with monitoring
+.claude/skills/ralph-runner/scripts/start.sh --monitor
+
+# Commit changes
+.claude/skills/ralph-runner/scripts/commit.sh "feat(module): description"
+```
+
+## Integration with Claude Code
+
+This skill is designed to be invoked when:
+1. User requests BtToxin Pipeline development
+2. User wants to start Ralph loop
+3. User needs to check development status
+4. User wants to continue interrupted development
+
+## Skill Metadata
+
+- **Name**: ralph-runner
+- **Description**: BtToxin Pipeline development with Ralph autonomous AI agent
+- **Actions**: start, monitor, status, commit, push
+- **Domain**: bttiaw.hzau.edu.cn
+
+## Related Files
+
+- `/vol1/1000/docker_server/traefik/web/zly/PROMPT.md` - Ralph instructions
+- `/vol1/1000/docker_server/traefik/web/zly/@fix_plan.md` - Task list
+- `/vol1/1000/docker_server/traefik/web/zly/specs/requirements.md` - Technical specs
+- `/vol1/1000/docker_server/traefik/web/zly/@AGENT.md` - Build commands
diff --git a/.claude/skills/ralph-runner/SKILL.md b/.claude/skills/ralph-runner/SKILL.md
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+---
+name: ralph-runner
+description: "BtToxin Pipeline development with Ralph autonomous AI agent. Actions: start, monitor, stop, status, commit, push. Features: 16-task concurrency, genome/protein analysis, i18n support, Docker deployment, CRISPR-Cas预留. Domain: bttiaw.hzau.edu.cn"
+---
+
+# Ralph Runner - BtToxin Pipeline Development
+
+Autonomous AI-powered development using Ralph for the BtToxin Pipeline project.
+
+## Prerequisites
+
+Check if Ralph is installed:
+
+```bash
+which ralph || ls ~/.local/bin/ralph-claude-code/ralph_loop.sh
+```
+
+If Ralph is not installed, install it:
+
+```bash
+~/.local/bin/claude-switch/tools/ralph-install-external.sh
+```
+
+Verify Ralph project files exist:
+
+```bash
+ls -la PROMPT.md @fix_plan.md specs/requirements.md
+```
+
+---
+
+## How to Use This Skill
+
+When user requests BtToxin Pipeline development (implement, build, fix, add, create), follow this workflow:
+
+### Step 1: Check Current Project Status
+
+```bash
+# Check git status
+git status
+
+# Check Ralph status
+ralph --status
+
+# View current task list
+cat @fix_plan.md
+```
+
+### Step 2: Start Ralph Loop
+
+**Recommended: With monitoring dashboard**
+```bash
+ralph --monitor
+```
+
+**Without monitoring (background process)**
+```bash
+ralph
+```
+
+**With call limit**
+```bash
+ralph --monitor --calls 50  # Limit to 50 calls per hour
+```
+
+### Step 3: Monitor Progress
+
+```bash
+# Check status
+ralph --status
+
+# View logs
+tail -f logs/ralph.log
+
+# Check response analysis
+cat status.json | jq .
+```
+
+### Step 4: Manual Operations (when needed)
+
+**Commit changes**
+```bash
+git add .
+git commit -m "feat(module): descriptive message"
+```
+
+**Push to remote**
+```bash
+git push
+```
+
+**Reset Ralph circuit breaker**
+```bash
+ralph --reset-circuit
+```
+
+**Check circuit breaker status**
+```bash
+ralph --circuit-status
+```
+
+---
+
+## Ralph Configuration
+
+### Environment Variables
+
+```bash
+# Set provider (glm, minimax, local, etc.)
+export CLAUDE_PROVIDER=minimax
+
+# Set model
+export CLAUDE_MODEL=MiniMax-M2.1
+
+# Set max iterations
+export RALPH_MAX_ITERATIONS=20
+```
+
+### Ralph Home Directory
+
+- **Commands**: `~/.local/bin/`
+- **Templates**: `~/.ralph/templates/`
+- **Scripts**: `~/.ralph/`
+
+---
+
+## Ralph Project Files
+
+| File | Purpose |
+|------|---------|
+| `PROMPT.md` | Ralph development instructions (REQUIRED) |
+| `@fix_plan.md` | Prioritized task list |
+| `specs/requirements.md` | Technical specifications |
+| `@AGENT.md` | Build/test/deploy commands |
+
+---
+
+## Common Workflows
+
+### Workflow 1: Start New Development Session
+
+```bash
+# 1. Pull latest changes
+git pull
+
+# 2. Check Ralph status
+ralph --status
+
+# 3. Start monitoring
+ralph --monitor
+```
+
+### Workflow 2: Review Completed Work
+
+```bash
+# 1. Check git log
+git log --oneline -10
+
+# 2. View changed files
+git diff HEAD~1
+
+# 3. Check @fix_plan.md progress
+grep -E '^\[.\]' @fix_plan.md | wc -l
+```
+
+### Workflow 3: Continue After Interruption
+
+```bash
+# 1. Check status
+ralph --status
+
+# 2. Resume if needed
+ralph  # or ralph --monitor
+```
+
+### Workflow 4: Emergency Stop
+
+```bash
+# Ctrl+C to stop monitoring
+# Or kill process
+pkill -f ralph_loop.sh
+```
+
+---
+
+## Project-Specific Guidelines
+
+### Current Development Priority
+
+Based on @fix_plan.md, current priorities are:
+
+1. **P0 - Frontend i18n**: ✅ Completed (vue-i18n, navigation, pages)
+2. **P0 - Upload Enhancement**: ✅ Completed (single file, genome/protein toggle)
+3. **P0 - Task Status**: ✅ Completed (queue status, progress)
+4. **P1 - FastAPI Backend**: Next focus
+5. **P1 - Concurrency Control**: Next focus
+6. **P2 - Docker Deployment**: Later focus
+
+### Completed Features
+
+- [x] Vue 3 frontend with Element Plus
+- [x] vue-i18n (Chinese/English)
+- [x] Navigation bar (Home, About, Submit, Status, Tool Guide)
+- [x] File upload (.fna/.fa/.fasta/.faa)
+- [x] Single file restriction
+- [x] Genome/Protein toggle
+- [x] Task status pages
+- [x] Tool Info page (Shoter methodology, no Digger mentioned)
+
+### Pending Features
+
+- [ ] FastAPI backend (main.py, tasks.py)
+- [ ] 16 concurrent task limit
+- [ ] Redis task queue
+- [ ] API endpoints (/api/tasks, /api/tasks/{id})
+- [ ] Docker deployment
+- [ ] Traefik configuration
+- [ ] CRISPR-Cas预留 (crispr_cas/ directory)
+
+---
+
+## Testing Guidelines
+
+Ralph follows these testing rules:
+
+1. **Testing effort**: ~20% of total effort
+2. **Priority**: Implementation > Documentation > Tests
+3. **Coverage**: 85% for new code
+4. **Focus**: Core functionality first
+
+---
+
+## Troubleshooting
+
+### "Prompt file 'PROMPT.md' not found"
+
+```bash
+# Ensure you're in the project directory
+cd /vol1/1000/docker_server/traefik/web/zly
+ls -la PROMPT.md
+```
+
+### "Ralph not installed"
+
+```bash
+# Install Ralph
+~/.local/bin/claude-switch/tools/ralph-install-external.sh
+```
+
+### Ralph stuck in loop
+
+```bash
+# Reset circuit breaker
+ralph --reset-circuit
+
+# Check circuit status
+ralph --circuit-status
+```
+
+### tmux session lost
+
+```bash
+# List sessions
+tmux list-sessions
+
+# Reconnect
+tmux attach -t ralph-watch
+
+# Or create new session
+tmux new -s ralph-watch
+ralph-monitor
+```
+
+---
+
+## Project Structure
+
+```
+web/zly/
+├── PROMPT.md              # Ralph instructions
+├── @fix_plan.md           # Task list
+├── @AGENT.md              # Build commands
+├── specs/
+│   └── requirements.md    # Tech specs
+├── frontend/              # Vue 3 SPA
+│   ├── src/
+│   │   ├── locales/       # i18n files
+│   │   ├── views/         # Pages
+│   │   └── components/    # Components
+│   └── package.json
+├── web/backend/           # FastAPI (to be built)
+├── scripts/               # Pipeline scripts
+├── docker/                # Docker configs
+└── external_dbs/          # BtToxin database
+```
+
+---
+
+## Deployment Targets
+
+| Target | URL | Status |
+|--------|-----|--------|
+| Development | localhost:5173/8000 | ✅ Frontend ready |
+| Test | bttiaw.hzau.edu.cn | 🔄 In progress |
+| Production | bttiaw.hzau.edu.cn | ⏳ Pending |
+
+---
+
+## Key Contacts
+
+- **Project**: BtToxin Pipeline
+- **Domain**: bttiaw.hzau.edu.cn
+- **Maintainer**: Claude Code with Ralph
diff --git a/.claude/skills/ralph-runner/scripts/commit.sh b/.claude/skills/ralph-runner/scripts/commit.sh
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+#!/bin/bash
+# Git Commit Helper
+# Usage: ./commit.sh "commit message"
+
+PROJECT_DIR="/vol1/1000/docker_server/traefik/web/zly"
+cd "$PROJECT_DIR"
+
+MESSAGE="${1:-"chore: update changes"}"
+
+echo "📝 Committing changes..."
+echo "Message: $MESSAGE"
+echo ""
+
+# Show staged files
+echo "📁 Files to commit:"
+git diff --cached --name-only
+echo ""
+
+# Commit
+git commit -m "$MESSAGE
+
+Co-Authored-By: Claude <noreply@anthropic.com>"
+
+echo ""
+echo "✅ Commit successful"
+echo "📊 Recent commits:"
+git log --oneline -3
diff --git a/.claude/skills/ralph-runner/scripts/start.sh b/.claude/skills/ralph-runner/scripts/start.sh
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+#!/bin/bash
+# Ralph Start Script
+# Usage: ./start.sh [--monitor] [--calls N]
+
+PROJECT_DIR="/vol1/1000/docker_server/traefik/web/zly"
+cd "$PROJECT_DIR"
+
+MONITOR=false
+CALLS=""
+
+# Parse arguments
+while [[ $# -gt 0 ]]; do
+    case $1 in
+        --monitor)
+            MONITOR=true
+            shift
+            ;;
+        --calls)
+            CALLS="--calls $2"
+            shift 2
+            ;;
+        *)
+            shift
+            ;;
+    esac
+done
+
+echo "🚀 Starting Ralph for BtToxin Pipeline..."
+echo ""
+
+# Pull latest changes first
+echo "📥 Pulling latest changes..."
+git pull
+
+# Check Ralph files
+echo "📁 Checking Ralph project files..."
+for f in PROMPT.md @fix_plan.md specs/requirements.md; do
+    if [ ! -f "$f" ]; then
+        echo "❌ Missing: $f"
+        exit 1
+    fi
+done
+echo "✅ All Ralph files present"
+
+# Start Ralph
+echo ""
+if [ "$MONITOR" = true ]; then
+    echo "📊 Starting Ralph with monitoring..."
+    ralph --monitor $CALLS
+else
+    echo "🚀 Starting Ralph..."
+    ralph $CALLS
+fi
diff --git a/.claude/skills/ralph-runner/scripts/status.sh b/.claude/skills/ralph-runner/scripts/status.sh
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+#!/bin/bash
+# Ralph Status Checker
+# Usage: ./status.sh
+
+PROJECT_DIR="/vol1/1000/docker_server/traefik/web/zly"
+cd "$PROJECT_DIR"
+
+echo "=== BtToxin Pipeline Ralph Status ==="
+echo ""
+
+# Git status
+echo "📊 Git Status:"
+git status --short | head -10
+echo ""
+
+# Ralph status
+echo "🤖 Ralph Status:"
+if command -v ralph &> /dev/null; then
+    ralph --status 2>/dev/null || echo "Ralph not running"
+else
+    echo "Ralph not installed"
+fi
+echo ""
+
+# Recent commits
+echo "📝 Recent Commits:"
+git log --oneline -5
+echo ""
+
+# Task progress
+echo "✅ Task Progress (from @fix_plan.md):"
+total=$(grep -c '^\[.\]' @fix_plan.md 2>/dev/null || echo "0")
+completed=$(grep -c '^\[x\]' @fix_plan.md 2>/dev/null || echo "0")
+echo "Completed: $completed / $total"
+echo ""
+
+# File checks
+echo "📁 Ralph Project Files:"
+for f in PROMPT.md @fix_plan.md @AGENT.md specs/requirements.md; do
+    if [ -f "$f" ]; then
+        echo "  ✅ $f"
+    else
+        echo "  ❌ $f (missing)"
+    fi
+done