diff --git a/backend/app/main_spa.py b/backend/app/main_spa.py
new file mode 100644
index 0000000..c2b4a6d
--- /dev/null
+++ b/backend/app/main_spa.py
@@ -0,0 +1,72 @@
+"""FastAPI 主应用 - with SPA static file serving"""
+from fastapi import FastAPI
+from fastapi.middleware.cors import CORSMiddleware
+from fastapi.staticfiles import StaticFiles
+from fastapi.responses import FileResponse
+from pathlib import Path
+from contextlib import asynccontextmanager
+
+from .config import settings
+from .api.v1 import jobs, upload, results, tasks
+
+
+@asynccontextmanager
+async def lifespan(app: FastAPI):
+    """应用生命周期管理"""
+    # 启动时
+    print("🚀 Starting BtToxin Pipeline API...")
+    yield
+    # 关闭时
+    print("👋 Shutting down BtToxin Pipeline API...")
+
+
+app = FastAPI(
+    title=settings.APP_NAME,
+    version=settings.APP_VERSION,
+    description="Automated Bacillus thuringiensis toxin mining pipeline",
+    lifespan=lifespan
+)
+
+# CORS
+app.add_middleware(
+    CORSMiddleware,
+    allow_origins=settings.CORS_ORIGINS,
+    allow_credentials=True,
+    allow_methods=["*"],
+    allow_headers=["*"],
+)
+
+# 路由
+app.include_router(jobs.router, prefix=f"{settings.API_V1_STR}/jobs", tags=["jobs"])
+app.include_router(tasks.router, prefix=f"{settings.API_V1_STR}/tasks", tags=["tasks"])
+app.include_router(upload.router, prefix=f"{settings.API_V1_STR}/upload", tags=["upload"])
+app.include_router(results.router, prefix=f"{settings.API_V1_STR}/results", tags=["results"])
+
+
+@app.get("/")
+async def root():
+    return {
+        "name": settings.APP_NAME,
+        "version": settings.APP_VERSION,
+        "status": "healthy"
+    }
+
+
+@app.get("/health")
+async def health():
+    return {"status": "ok"}
+
+
+# Mount static files for frontend (in production)
+# The frontend dist files are served from /app/frontend/dist in the container
+frontend_dist_path = Path("/app/frontend/dist")
+if frontend_dist_path.exists():
+    app.mount("/assets", StaticFiles(directory=str(frontend_dist_path / "assets")), name="assets")
+
+    @app.get("/{full_path:path}", include_in_schema=False)
+    async def serve_spa(full_path: str):
+        """Serve frontend SPA - all routes go to index.html"""
+        file_path = frontend_dist_path / full_path
+        if file_path.exists() and file_path.is_file():
+            return FileResponse(file_path)
+        return FileResponse(frontend_dist_path / "index.html")
diff --git a/docker/dockerfiles/Dockerfile.traefik b/docker/dockerfiles/Dockerfile.traefik
index 489ae23..6e729b1 100644
--- a/docker/dockerfiles/Dockerfile.traefik
+++ b/docker/dockerfiles/Dockerfile.traefik
@@ -14,7 +14,7 @@ COPY pixi.toml .
 COPY pyproject.toml .
 COPY scripts/ scripts/
 COPY bttoxin/ bttoxin/
-COPY web/ web/
+COPY backend/ backend/
 COPY Data/ Data/
 
 # Install all pixi environments
@@ -31,8 +31,7 @@ FROM node:20 AS frontend-builder
 
 WORKDIR /app
 
-# Copy frontend source from builder
-COPY --from=builder /app/web ../web/
+# Copy frontend source
 COPY frontend/ .
 
 RUN npm install -g pnpm && \
@@ -60,7 +59,7 @@ COPY --from=builder /app/.pixi /app/.pixi
 COPY --from=builder /shell-hook.sh /shell-hook.sh
 
 # Copy backend code
-COPY --from=builder /app/web /app/web
+COPY --from=builder /app/backend /app/backend
 COPY --from=builder /app/Data /app/Data
 COPY --from=builder /app/bttoxin /app/bttoxin
 COPY --from=builder /app/scripts /app/scripts
@@ -77,11 +76,12 @@ EXPOSE 8000
 
 # Health check
 HEALTHCHECK --interval=30s --timeout=10s --start-period=5s --retries=3 \
-    CMD curl -f http://localhost:8000/api/health || exit 1
+    CMD curl -f http://localhost:8000/health || exit 1
 
 # Entrypoint
 ENTRYPOINT ["/bin/bash", "/shell-hook.sh"]
 
 # Command: Start FastAPI backend
 # FastAPI will serve both API and frontend static files
-CMD ["uvicorn", "web.backend.main:app", "--host", "0.0.0.0", "--port", "8000"]
+# Note: Using backend.app.main_spa for SPA static file support
+CMD ["uvicorn", "backend.app.main_spa:app", "--host", "0.0.0.0", "--port", "8000"]